The lyrics of "A Mouse Analyzing Just One," were written in 2016 by Dennis Pearl from Penn State University. The song may be sung to the tune of the traditional folk song, "The House of the Rising Sun," popularized by the British Rock band the Animals in 1964. The song is meant to convey the problems with using biological material from a single animal in experiments. Audio of the parody was produced and sung by students in the commercial music program of The University of Teas at El Paso.